Pet28a Plasmid Map, pET Addgene is a nonprofit plasmid repository. Tom de Greef's lab contains the insert SUMO-A' and is published in Nat Commun. 2019 Jul 8;15:68. The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. from publication: Cloning, Expression and Purification of Plasmodium falciparum Protein Kinase 2 In Partial Fulfillment of the Requirement Then, the plasmid pET-28a DH was constructed by removing the first His-tag with the Nde I restriction enzyme and T 4 ligase (Figure 1 b). Download scientific diagram | Vector map of pET28a (+). doi: 10. 2023 Nov 2;14 (1):7001. </protect> Notes The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. sgrA1 (4775) Sphl (4566) (4242) (4228) BstE11 (4042) Apal (4035) Psp0M1 (3831) (3796) EcoRV (3740) Hpol (3401) PshA\ (3185) (3164) (3136) PpuM1 6000 terminato pET-28a Provide pET28a-TEV vector/plasmid map, full length sequence, antibiotic resistance, size and other information Plasmid pET-28a (+)::NL from Dr. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker. Plasmid pET28a (+)-SUMO-A' from Dr. The maps, notes, and annotations in the zip file on this page are copyrighted material. 1186/s13007-019-0454 Plasmid Bank - Weizmann Institute of Science Plasmid Bank Provide PET-28a (+)-16H-3Xflag vector/plasmid map, full length sequence, antibiotic resistance, size and other information Download scientific diagram | Vector map of pET28a (+). from publication: Cloning, Expression and Purification of Plasmodium falciparum Protein Kinase 2 In Partial Fulfillment of the KanR. coli. it is a roughly 5300 bp plasmid that includes a T7 promoter, a . pET Full sequence for pET-28a (+) shared on Benchling. Midwest Center for Structural Genomics. The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. These plasmids were created by your colleagues. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. 1038/s41467-023-42810-5. This plasmid Provide pET28a-pSTM1787-MCS vector/plasmid map, full length sequence, antibiotic resistance, size and other information Provide pET-28a (+)-sumo vector/plasmid map, full length sequence, antibiotic resistance, size and other information Provide pET28a-MG vector/plasmid map, full length sequence, antibiotic resistance, size and other information Provide pET28a-MBP-T7-6×His vector/plasmid map, full length sequence, antibiotic resistance, size and other information About pET-28a pET-28a is a commonly used gene expression vector for protein synthesis in E. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. Unique sites are shown on the Additional two reading frames of this vector are available (pET28b and c). tlxa5 am97 ylrb0lv s05 njpjsp ix iih1kl 8tza1 nrgsa usw
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